Afnan Shirbini

Conducted long-term independent research on DNA replication using the SV40 replication system, focusing on protein–DNA interactions investigated through in vitro biochemical and single-molecule approaches.
Designed, executed, and troubleshot molecular biology and biochemical experiments, including cloning, PCR, protein expression and purification, and functional assays supporting mechanistic studies.
Performed protein purification and biochemical characterisation using affinity, ion-exchange, and size-exclusion chromatography, including operation of AKTA FPLC systems.
Utilized advanced fluorescence microscopy (TIRF) and quantitative imaging tools to analyze molecular interactions and dynamic biological processes.
Performed biochemical and biophysical assays, including Western blotting, EMSA, and microscale thermophoresis (MST), to assess protein quality, binding affinity, and molecular interactions.
Optimized and adapted experimental protocols to improve data quality, reproducibility, and overall assay performance.
Maintained detailed experimental records, raw data files, and laboratory notebooks in compliance with research integrity and safety standards.
Conducted data analysis and interpretation using ImageJ, OriginLab, and statistical approaches to support scientific conclusions.
Prepared figures, data summaries, written reports, and scientific presentations for internal lab meetings and collaborative discussions.
Contributed to manuscript preparation and revisions, resulting in multiple peer-reviewed publications in high-impact journals.
Collaborated with international research partners, supporting cross-institutional projects and shared experimental workflows.
Ensured laboratory organisation, safety compliance, and proper handling of hazardous materials, including radioactive reagents, under approved protocols.