Nikita Pinjarkar

Gained expertise in NGS methodologies, including sequence alignment, gene prediction, and variant calling.
Performed genomic data analysis using BLAST, FASTA, and multiple sequence alignment techniques while designing primers for PCR and real-time PCR experiments.
Conducted RNA-Seq analysis, gene expression profiling, alternative splicing studies, and variant analysis. Performed NGS data processing, quality evaluation, and alignment theories, improving seq alignment accuracy by 15%.
Leveraged GenMark & GenScan for genome annotation, resolved misannotations, explored de novo assembly, ChIP-seq analysis, and metagenomics sequencing for precise genomic analysis.
Developed bioinformatics workflows for large-scale genomic studies, ensuring high-quality data processing and interpretation.

Extracted & purified DNA from a fluorescent fish gene to analyze gene expression and protein function.
Performed PCR amplification, agarose gel electrophoresis, and cloning, followed by cell culture for recombinant protein expression and analysis like affinity and size-exclusion chromatography for downstream analysis.
Utilized SDS-PAGE and fluorescence microscopy to assess protein expression, integrity, and localization.
Applied BLAST for sequence alignment and comparative genomic analysis, gaining hands-on experience in molecular techniques.

Performed clinical diagnostics in haematology (CBC, blood smear preparation, ESR, coagulation tests), microbiology, biochemistry assays (blood glucose, lipid profile, liver and kidney function tests), and serology.
Conducted microbiological techniques, including Gram staining, culture methods, and antibiotic sensitivity testing.
Executed serological tests such as ELISA, Widal, VDRL, and CRP, along with urine and stool analysis for clinical diagnostics.
Operated automated lab equipment, ensured quality control, and managed patient sample processing for accurate reporting.