Giovanni Colombo

Biophysical characterization of proteins undergoing liquid-liquid phase separation (LLPS).
In vitro and in cellulo studies of proteins related to epithelial-to-mesenchymal transition (EMT) and metastatic processes.
Developing protocols for exosome purification and characterization.

Developing a protocol for generation of hESC- and hiPSC-derived organoids in ultra-low attachment plates and in DASbox mini bioreactor (Eppendorf).
Differentiation of hiPSCs (including hiPSCs-KS) and hESCs into neural precursor lineages.
Studies on DNA demethylases involved in neural and cardiac stem cells differentiation.

Testing new protocols for the generation of pancreatic β-cells in vitro.
Investigating the role of high atmospheric pressure on stem cell maintenance.

Analysing genetic mutations and enzymatic alteration in Anderson-Fabry, Gaucher and Pompe disease (Lysosomal Storage Diseases, LSDs).
Investigating the role of specific microRNAs as biomarkers in LSDs.

Evaluating specific exosomal microRNAs as biomarker for the detection of LSDs.

Investigating the role of proteins involved in the epithelial-mesenchymal transition (EMT) of colorectal cancer.

Studying genetic mutations and enzymatic alteration in patient affected by Anderson-Fabry.

Investigating genetic mutations and phenotype correlation in Anderson-Fabry disease.